全文获取类型
收费全文 | 2053篇 |
免费 | 132篇 |
国内免费 | 28篇 |
出版年
2024年 | 3篇 |
2023年 | 17篇 |
2022年 | 33篇 |
2021年 | 61篇 |
2020年 | 50篇 |
2019年 | 57篇 |
2018年 | 59篇 |
2017年 | 49篇 |
2016年 | 63篇 |
2015年 | 63篇 |
2014年 | 156篇 |
2013年 | 173篇 |
2012年 | 109篇 |
2011年 | 162篇 |
2010年 | 138篇 |
2009年 | 124篇 |
2008年 | 122篇 |
2007年 | 108篇 |
2006年 | 97篇 |
2005年 | 72篇 |
2004年 | 88篇 |
2003年 | 69篇 |
2002年 | 40篇 |
2001年 | 28篇 |
2000年 | 20篇 |
1999年 | 24篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 18篇 |
1995年 | 20篇 |
1994年 | 15篇 |
1993年 | 13篇 |
1992年 | 13篇 |
1991年 | 18篇 |
1990年 | 10篇 |
1989年 | 12篇 |
1988年 | 12篇 |
1987年 | 8篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 7篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1973年 | 5篇 |
排序方式: 共有2213条查询结果,搜索用时 15 毫秒
991.
Elevated baseline corticosterone levels function to mobilize energy in predictable life-history stages, such as bird migration. At the same time, baseline corticosterone has a permissive effect on the accumulation of fat stores (fueling) needed for migratory flight. Most migrants alternate flight bouts with stopovers, during which they replenish the fuel used during the preceding flight (refueling). The role of corticosterone in refueling is currently unclear. In a fasting–re-feeding experiment on northern wheatears (Oenanthe oenanthe) in autumn we found that baseline total and free corticosterone levels were negatively related with both food intake and the rate of fuel deposition after fasting. This confirms our earlier findings in wild conspecifics in spring and indicates that corticosterone does not stimulate stopover refueling. Whether the negative relationship between baseline corticosterone level and fuel deposition rate is causal is questionable, because within-individual comparison of corticosterone metabolite levels in droppings did not reveal differences between refueling and control periods. In other words, corticosterone does not appear to be down-regulated during refueling, which would be expected if it directly hampers refueling. We discuss possible correlates of corticosterone level that may explain the negative association between corticosterone and stopover refueling. Additionally, we found that fasting decreases total corticosterone level, which contrasts with previous studies. We propose that the difference is due to the other studies being conducted outside of the migration life-history stage, and provide a possible explanation for the decrease in corticosterone during fasting in migrating birds. 相似文献
992.
While the spread of Toxoplasma gondii within the infected human or animal host is associated with pathology, the pathways of dissemination have remained enigmatic. From the time point of entry into the gut, to the quiescent chronic infection in the central nervous system, Toxoplasma is detected and surveyed by immune cells that populate the tissues, for example dendritic cells. Paradoxically, this protective migratory function of leukocytes appears to be targeted by Toxoplasma to mediate its dissemination in the organism. Recent findings show that tightly regulated events take place shortly after host cell invasion that promote the migratory activation of infected dendritic cells. Here, we review the emerging knowledge on how this obligate intracellular protozoan orchestrates the subversion of leukocytes to achieve systemic dissemination and reach peripheral organs where pathology manifests. 相似文献
993.
Jie Wang Jinlei Song Chao An Wenji Dong Jingxin Zhang Changcheng Yin John Hale Anthony J. Baines Narla Mohandas Xiuli An 《The Journal of biological chemistry》2014,289(9):5925-5937
Protein 4.1B is a member of protein 4.1 family, adaptor proteins at the interface of membranes and the cytoskeleton. It is expressed in most mammalian tissues and is known to be required in formation of nervous and cardiac systems; it is also a tumor suppressor with a role in metastasis. Here, we explore functions of 4.1B using primary mouse embryonic fibroblasts (MEF) derived from wild type and 4.1B knock-out mice. MEF cells express two 4.1B isoforms: 130 and 60-kDa. 130-kDa 4.1B was absent from 4.1B knock-out MEF cells, but 60-kDa 4.1B remained, suggesting incomplete knock-out. Although the 130-kDa isoform was predominantly located at the plasma membrane, the 60-kDa isoform was enriched in nuclei. 130-kDa-deficient 4.1B MEF cells exhibited impaired cell adhesion, spreading, and migration; they also failed to form actin stress fibers. Impaired cell spreading and stress fiber formation were rescued by re-expression of the 130-kDa 4.1B but not the 60-kDa 4.1B. Our findings document novel, isoform-selective roles for 130-kDa 4.1B in adhesion, spreading, and migration of MEF cells by affecting actin organization, giving new insight into 4.1B functions in normal tissues as well as its role in cancer. 相似文献
994.
Seow Theng Ong Michael Freeley Joanna Skubis-Zegad?o Mobashar Hussain Urf Turabe Fazil Dermot Kelleher Friedrich Fresser Gottfried Baier Navin Kumar Verma Aideen Long 《The Journal of biological chemistry》2014,289(28):19420-19434
Rab GTPases control membrane traffic and receptor-mediated endocytosis. Within this context, Rab5a plays an important role in the spatial regulation of intracellular transport and signal transduction processes. Here, we report a previously uncharacterized role for Rab5a in the regulation of T-cell motility. We show that Rab5a physically associates with protein kinase Cϵ (PKCϵ) in migrating T-cells. After stimulation of T-cells through the integrin LFA-1 or the chemokine receptor CXCR4, Rab5a is phosphorylated on an N-terminal Thr-7 site by PKCϵ. Both Rab5a and PKCϵ dynamically interact at the centrosomal region of migrating cells, and PKCϵ-mediated phosphorylation on Thr-7 regulates Rab5a trafficking to the cell leading edge. Furthermore, we demonstrate that Rab5a Thr-7 phosphorylation is functionally necessary for Rac1 activation, actin rearrangement, and T-cell motility. We present a novel mechanism by which a PKCϵ-Rab5a-Rac1 axis regulates cytoskeleton remodeling and T-cell migration, both of which are central for the adaptive immune response. 相似文献
995.
996.
Hee-Jun Kim Jae-Gyu Kim Mi-Young Moon Seol-Hye Park Jae-Bong Park 《The Journal of biological chemistry》2014,289(3):1429-1440
997.
Vladislav S. Golubkov Natalie L. Prigozhina Yong Zhang Konstantin Stoletov John D. Lewis Phillip E. Schwartz Robert M. Hoffman Alex Y. Strongin 《The Journal of biological chemistry》2014,289(35):24238-24249
It is well established that widely expressed PTK7 is essential for vertebrate tissue morphogenesis. In cancer, the functionality of PTK7 is selectively regulated by membrane type-1 matrix metalloproteinase (MT1-MMP), ADAMs (a
disintegrin domain and metalloproteinases), and γ-secretase proteolysis. Here, we established that the full-length membrane PTK7, its Chuzhoi mutant with the two functional MT1-MMP cleavage sites, and its L622D mutant with the single inactivated MT1-MMP cleavage site differentially regulate cell motility in a two-dimensional versus three-dimensional environment. We also demonstrated that in polarized cancer cells, the levels of PTK7 expression and proteolysis were directly linked to the structure and kinetics of cell protrusions, including lamellipodia and invadopodia. In the functionally relevant and widely accepted animal models of metastasis, mouse and chick embryo models, both the overexpression and knock-out of PTK7 in HT1080 cells abrogated metastatic dissemination. Our analysis of human tissue specimens confirmed intensive proteolysis of PTK7 in colorectal cancer tumors, but not in matching normal tissue. Our results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer. 相似文献
998.
Chakrabhavi Dhananjaya Mohan Hanumantharayappa Bharathkumar Krishna C. Bulusu Vijay Pandey Shobith Rangappa Julian E. Fuchs Muthu K. Shanmugam Xiaoyun Dai Feng Li Amudha Deivasigamani Kam M. Hui Alan Prem Kumar Peter E. Lobie Andreas Bender Basappa Gautam Sethi Kanchugarakoppal S. Rangappa 《The Journal of biological chemistry》2014,289(49):34296-34307
999.
Hee Jun Cho Yoo-Seok Hwang Kathleen Mood Yon Ju Ji Junghwa Lim Deborah K. Morrison Ira O. Daar 《The Journal of biological chemistry》2014,289(26):18556-18568
The Eph receptors and their membrane-bound ligands, ephrins, play important roles in various biological processes such as cell adhesion and movement. The transmembrane ephrinBs transduce reverse signaling in a tyrosine phosphorylation-dependent or -independent, as well as PDZ-dependent manner. Here, we show that ephrinB1 interacts with Connector Enhancer of KSR1 (CNK1) in an EphB receptor-independent manner. In cultured cells, cotransfection of ephrinB1 with CNK1 increases JNK phosphorylation. EphrinB1/CNK1-mediated JNK activation is reduced by overexpression of dominant-negative RhoA. Overexpression of CNK1 alone is sufficient for activation of RhoA; however, both ephrinB1 and CNK1 are required for JNK phosphorylation. Co-immunoprecipitation data showed that ephrinB1 and CNK1 act as scaffold proteins that connect RhoA and JNK signaling components, such as p115RhoGEF and MKK4. Furthermore, adhesion to fibronectin or active Src overexpression increases ephrinB1/CNK1 binding, whereas blocking Src activity by a pharmacological inhibitor decreases not only ephrinB1/CNK1 binding, but also JNK activation. EphrinB1 overexpression increases cell motility, however, CNK1 depletion by siRNA abrogates ephrinB1-mediated cell migration and JNK activation. Moreover, Rho kinase inhibitor or JNK inhibitor treatment suppresses ephrinB1-mediated cell migration. Taken together, our findings suggest that CNK1 is required for ephrinB1-induced JNK activation and cell migration. 相似文献
1000.
Volker K?nigs Richard Jennings Thomas Vogl Markus Horsthemke Anne C. Bachg Yan Xu Kay Grobe Cord Brakebusch Albrecht Schwab Martin B?hler Ulla G. Knaus Peter J. Hanley 《The Journal of biological chemistry》2014,289(44):30772-30784
RhoA is thought to be essential for coordination of the membrane protrusions and retractions required for immune cell motility and directed migration. Whether the subfamily of Rho (Ras homolog) GTPases (RhoA, RhoB, and RhoC) is actually required for the directed migration of primary cells is difficult to predict. Macrophages isolated from myeloid-restricted RhoA/RhoB (conditional) double knock-out (dKO) mice did not express RhoC and were essentially “pan-Rho”-deficient. Using real-time chemotaxis assays, we found that retraction of the trailing edge was dissociated from the advance of the cell body in dKO cells, which developed extremely elongated tails. Surprisingly, velocity (of the cell body) was increased, whereas chemotactic efficiency was preserved, when compared with WT macrophages. Randomly migrating RhoA/RhoB dKO macrophages exhibited multiple small protrusions and developed large “branches” due to impaired lamellipodial retraction. A mouse model of peritonitis indicated that monocyte/macrophage recruitment was, surprisingly, more rapid in RhoA/RhoB dKO mice than in WT mice. In comparison with dKO cells, the phenotypes of single RhoA- or RhoB-deficient macrophages were mild due to mutual compensation. Furthermore, genetic deletion of RhoB partially reversed the motility defect of macrophages lacking the RhoGAP (Rho GTPase-activating protein) myosin IXb (Myo9b). In conclusion, the Rho subfamily is not required for “front end” functions (motility and chemotaxis), although both RhoA and RhoB are involved in pulling up the “back end” and resorbing lamellipodial membrane protrusions. Macrophages lacking Rho proteins migrate faster in vitro, which, in the case of the peritoneum, translates to more rapid in vivo monocyte/macrophage recruitment. 相似文献